产品详情
产品名称Merlin Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total Merlin protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.516~520 (R-L-S-M-E) derived from Human Merlin.
基因/蛋白名称Merlin
标记Unconjugated
别名MERL; NF2; Neurofibromin 2; SCH; Schwannomerlin
数据库入口号Swiss-Prot: P35240
NCBI Protein: NP_000259.1
Uniprot
P35240
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 69kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Mouse brain tissue and C6 cells using Merlin(Ab-518) Antibody #21258.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Merlin(Ab-518) Antibody #21258(left) or the same antibody preincubated with blocking peptide(right).
Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity.
Guang-Hui Xiao, et al. (2005) Mol. Cell. Biol ; 25: 2384 - 2394.
Hi-Su Yang, et al. (2006) Cancer Res ; 66: 2708 - 2715.
R Bohni, et al. (1994) J. Biol. Chem ; 269: 14541 - 14545.
Adam J. Ratner, et al. (2001) J. Biol. Chem ; 276: 19267 - 19275.
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et al,Autoantibody against Tumor-Associated Antigens as Diagnostic Biomarkers in Hispanic Patients with Hepatocellular Carcinoma. In Cells on 2022 Oct 14 by Yangcheng Ma, Cuipeng Qiu, et al..PMID:36291095
, (2022),
PMID:
36291095
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