免费电话:400-9961-933
邮箱:info@sabbiotech.com
欢迎光临本站
川博智能体 English
位置： 首页 > 多克隆抗体 > p70 S6 Kinase Antibody

p70 S6 Kinase Antibody#21276

评价
查看说明书
是否有货： 有货
产品详情 图片 产品描述 发表文献(7) 其它
产品详情

产品名称p70 S6 Kinase Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB;IHC;IF

种属反应性Human;Mouse;Rat

特异性The antibody detects endogenous level of total p70 S6 Kinase protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.422~426 (P-V-S-P-V) derived from Human p70S6k.

基因/蛋白名称p70 S6 Kinase

标记Unconjugated

别名KS6B1; P70-S6K; RPS6KB1; S6K;

数据库入口号Swiss-Prot: P23443
NCBI Protein: NP_003152.1

Uniprot P23443

计算分子量59kDa

实际分子量70,85kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
WB 1:500-1:2000; IHC 1:50-1:200; IF 1:50-1:200;
Western blot analysis of extracts from 293 cells using p70 S6 Kinase Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemistry of paraffin-embedded mouse lung using p70 S6 Kinase Antibody.
Immunohistochemistry of paraffin-embedded rat brain using p70 S6 Kinase Antibody.
Immunofluorescence staining of methanol-fixed Hela cells using p70 S6 Kinase Antibody.Blue: DAPI for nuclear staining.
Immunofluorescence staining of methanol-fixed Rat brain using p70 S6 Kinase Antibody.
Immunofluorescence staining of methanol-fixed HEK293 cells using p70 S6 Kinase Antibody(red).Picture A:Target. Picture B: DAPI (blue). Picture C: Merge of A+B.
Immunofluorescence analysis of U2OS cells using p70 S6 Kinase Antibody. Blue: DAPI for nuclear staining.

RPS6KB1 phosphorylates the Ribosomal Protein-S6. Activation of RPS6KB1 requires a complex, ordered series of conformational changes and phosphorylation reactions. While the role of sequential, multi-site phosphorylation has been extensively detailed, characterization of the priming step required to initiate this cascade has remained elusive. Probably this priming process is dependent on calcium. Calcium-dependent regulation of RPS6KB1 does not specifically target Thr-229 and Thr-389, the key regulatory phosphorylation sites; rather, calcium chelation results in a global inhibition of RPS6KB1 phosphorylation. The initial calcium-dependent process is required to release an inhibitory interaction between the C- and N-termini of RPS6KB1, thus allowing phosphorylation of key domains. The priming event involves formation of a calcium-dependent protein complex that releases the interaction between the N- and C-termini. RPS6KB1 is then accessible for activation by the kinases that target the known regulatory phosphorylation sites .

Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851
Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84.
Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c
Le, X.F, et al. (2003) Oncogene 22: 484

如果您使用该产品21276发表了文章，请通知我们，让我们可以引用您的文献。

et al,5-HT 2 receptor mediates high-fat diet-induced hepatic steatosis and very low density lipoprotein overproduction in rats.In Obes Res Clin Pract. On 2018 Jan - Feb by Li X, Guo K et al..PMID: 27133527 , (2018),
PMID: 27133527
et al,Effect of electrical stimulation combIned with diet therapy on InsulIn resistance via mTOR signalIng. In Mol Med Rep on 2019 Dec by Huang S, Tang N, et al..PMID:31702811 , (2019),
PMID: 31702811
et al,EBV-LMP1 Regulating AKT/mTOR Signaling Pathway and WWOX in Nasopharyngeal Carcinoma.In Int J Clin Exp Pathol on 2017 Aug 1 by Lingyan Qin, Xiaohong Li,et al..PMID: 31966718 , (2017),
PMID: 31966718
et al,Rapamycin enhances the anti-cancer effect of dasatinib by suppressing Src/PI3K/mTOR pathway in NSCLC cells. In PLoS One on 2015 Jun 10 by Bin Chen, Xin Xu,et al..PMID:26061184 , (2015),
PMID: 26061184
et al,Effective treatment with combination of peripheral 5鈥恏ydroxytryptamine synthetic inhibitor and 5鈥恏ydroxytryptamine 2 receptor antagonist on glucocorticoid鈥恑nduced whole鈥恇ody insulin resistance with hyperglycemia.In J Diabetes Investig on 2016 Nov by Shaoxin Ma , Tao Li et al..PMID:27177506 , (2016),
PMID: 27177506
et al,Long-term Stress with Hyperglucocorticoidemia-induced Hepatic Steatosis with VLDL Overproduction Is Dependent on both 5-HT2 Receptor and 5-HT Synthesis in Liver.In Int J Biol Sci on 2016 Jan 1 by Jihua Fu , Shaoxin Ma et al..PMID: 26884719 , (2016),
PMID: 26884719
et al,miR-223 reverses the resistance of EGFR-TKIs through IGF1R/PI3K/Akt signaling pathway.In Int J Oncol.On 2016 May by J Han, F Zhao et al..PMID: 26936292 , (2016),
PMID: 26936292

操作手册

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu人
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk猴
  • Rb兔
  • B牛
  • D狗
  • P猪
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk鸡
;

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

info@sabbiotech.com

帮助与技术支持

联系我们

订购帮助

条款

政策

技术支持

新闻动态

快速导航

公司介绍

品牌历史

细胞信号转导

癌症研究

干细胞研究

神经科学

代理商