产品详情
产品名称Smad2 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total Smad2 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.218~222 (P-E-T-P-P) derived from Human Smad2.
基因/蛋白名称Smad2
标记Unconjugated
别名JV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2
数据库入口号Swiss-Prot: Q15796
NCBI Protein: NP _001003652.1
Uniprot
Q15796
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 and 3T3 cells using Smad2(Ab-220) Antibody #21323
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad2(Ab-220) Antibody #21323(left) or the same antibody preincubated with blocking peptide(right).
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420
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et al,Distribution of Smad mRNA and proteins in the rat brain.In J Chem Neuroanat. On 2018 Jul by Nakajima T, Hata R et al..PMID: 29196107
, (2018),
PMID:
29196107
et al,TGF β1 mediated Smad signaling pathway and EMT in hepatic fibrosis induced by Nano NiO in vivo and in vitro.In Environ Toxicol on 2020 Apr by Zhang Q, Chang X ,et al..PMID: 31737983
, (2020),
PMID:
31737983
et al,Temporal and regional patterns of Smad activation in the rat hippocampus following global ischemia.In J Neurol Sci on 2014 Feb 15 by Takayuki Nakajima , Masafumi Yanagihara et al ..PMID:24290497
, (2014),
PMID:
24290497
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