产品详情
产品名称Caspase8 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB
种属反应性Human
特异性The antibody detects endogenous level of total Caspase 8 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.217~221 (S-E-S-Q-T) derived from Caspase 8.
基因/蛋白名称Caspase-8
标记Unconjugated
别名ICE-like apoptotic protease 5
数据库入口号Swiss-Prot: Q14790
NCBI Protein: NP_001219.2
Uniprot
Q14790
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 18,43,57kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from 293, Hela and HT29 cells using Caspase8 Antibody #21421
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Himeji D.et.al.(2002)Blood 99:4070-4078
Breckenridge D.G.et.al. (2002)Proc. Natl. Acad. Sci. U.S.A. 99:4331-4336
Muzio M.et.al. (1997)J. Biol. Chem. 272:2952-2956
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