产品详情
产品名称Nanog Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB
种属反应性Human;Mouse
特异性The antibody detects endogenous level of total Nanog protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.137~141 (K-Q-V-K-T) derived from Nano g
基因/蛋白名称Nanog
标记Unconjugated
别名Homeobox transcription factor Nanog
数据库入口号Swiss-Prot: Q9H9S0
NCBI Protein: NP_079141.2
Uniprot
Q9H9S0
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 42kd
Western blotting: 1:1000
Western blot analysis of extracts from human Umbilical cord mesenchymal stem cell using Nano g Antibody #21423.
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation
Do HJ, et al.Biochem Biophys Res Commun. 2007 Feb 16;353(3):770-5.
Boyer LA, et al.Cell. 2005 Sep 23;122(6):947-56.
Freberg CT, et al. Mol Biol Cell. 2007 May;18(5):1543-53.
Chambers I, et al. Cell. 2003 May 30;113(5):643-55.
如果您使用该产品21423发表了文章，请通知我们，让我们可以引用您的文献。
et al,Tumorigenic hybrids between mesenchymal stem cells and gastric cancer cells enhanced cancer proliferation, migration and stemness.In BMC Cancer on 2015 Oct 24 by Jianguo Xue , Yuan Zhu et al..PMID: 26498753
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et al,SALL4 promotes gastric cancer progression through activating CD44 expression.In Oncogenesis on 2016 Nov 7 by X Yuan , X Zhang et al..PMID:27819668
, (2016),
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27819668
et al,HucMSC exosome-delivered 14-3-3ζ orchestrates self-control of the Wnt response via modulation of YAP during cutaneous regeneration. In Stem Cells on 2016 Oct by Bin Zhang, Yinghong Shi, et al..PMID: 27334574
, (2016),
PMID:
27334574
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