产品详情
产品名称MEK1/2 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC;IF;IP;ELISA
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total MEK1/2 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.235-239(L-Q-G-T-H) derived from Human MEK1/2.
基因/蛋白名称MEK1/2
标记Unconjugated
别名MEK1; MKK1; MAPKK1; PRKMK1;
数据库入口号Swiss-Prot: Q02750
NCBI Protein: NP_002746.1
Uniprot
Q02750
计算分子量43, 44kDa
实际分子量44kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:1000-1:5000; IHC 1:50-1:1000; IF 1:50-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200;
Western blot analysis of extracts from various cell lines using MEK1/2 Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/2 Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using MEK1/2 Antibody.Negative control was used by secondary antibody only.
Immunofluorescence staining of methanol-fixed Hela cells using MEK1/2 Antibody(green).
Immunofluorescence staining of methanol-fixed HEK293 cells using MEK1/2 Antibody.Picture A: MEK1/2 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Seger R., Seger D., Lozeman F.J., Ahn N.G., Graves L.M., Campbell J.S., Ericsson L., Harrylock M., Jensen A.M., Krebs E.G.J. Biol. Chem. 267:25628-25631(1992)
Mukherjee S., Keitany G., Li Y., Wang Y., Ball H.L., Goldsmith E.J., Orth K.Science 312:1211-1214(2006)
如果您使用该产品21428发表了文章，请通知我们，让我们可以引用您的文献。
et al,Integrin 伪6/Akt/Erk signaling is essential for human breast cancer resistance to radiotherapy.In Sci Rep on 2016 Sep 14 by Ting Hu, Rui Zhou et al..PMID:27624978
, (2016),
PMID:
27624978
et al,MicroRNA锟?46b, a sensitive indicator of mesenchymal stem cell repair of acute renal injury.In Stem Cells Transl Med on 2016 Oct by Yuan Zhu, Jing Yu et al..PMID:27400799
, (2016),
PMID:
27400799
et al,7?dehydrocholesterol suppresses melanoma cell proliferation and invasion via Akt1/NF?kB signaling,In Oncol Lett on 2020 Dec by Jia Liu, Feiliang Zhong, et al..PMID: 33193858
, (2020),
PMID:
33193858
有货
