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位置: 首页 > 多克隆抗体 > CD44 Antibody

CD44 Antibody#21471

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产品详情

产品名称CD44 Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IF

种属反应性Human

特异性The antibody detects endogenous level of total CD44 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.734~738(Q-N-V-D-M) derived from Human CD44.

基因/蛋白名称CD44

标记Unconjugated

别名LHR; MDU2; MDU3; MIC4;

数据库入口号Swiss-Prot: P16070
NCBI Protein: NP_000601.3

Uniprot P16070

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunofluorescence:1:100~1:200
Western blot analysis of extract from Hela cells using CD44 Antibody #21471
Immunofluorescence staining of methanol-fixed NCI-h446 cells showing cytoplasmic staining using CD44 Antibody #21471.

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

Teriete P.,et.al(2004) Mol. Cell 13:483-496 Bartolazzi A. (2003)Melanoma Res. 13:325-337
Liu T.et.al (2005)J. Proteome Res. 4:2070-2080

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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