产品详情
产品名称AML1(RUNX1) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB
种属反应性Human
特异性The antibody detects endogenous level of total AML1(RUNX1) protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.6~10(D-A-S-T-S) derived from Human AML1(RUNX1).
基因/蛋白名称AML1(RUNX1)
标记Unconjugated
别名CBFA2; EVI-1; AMLCR1
数据库入口号Swiss-Prot: Q01196
NCBI Protein: NP_001116079.1
Uniprot
Q01196
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 55kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from P19, Hela and 293 cells using AML1(RUNX1) Antibody #21477
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The a subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D.Mol. Cell. Biol. 19:3635-3644(1999)
Pelletier N., Champagne N., Stifani S., Yang X.-J. Oncogene 21:2729-2740(2002)
Cho J.-Y., Akbarali Y., Zerbini L.F., Gu X., Boltax J., Wang Y., Oettgen P., Zhang D.-E., Libermann T.A.J. Biol. Chem. 279:19512-19522(2004)
如果您使用该产品21477发表了文章，请通知我们，让我们可以引用您的文献。
有货
