产品详情
产品名称Vimentin Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC;IF;ELISA
种属反应性Human;Mouse;Rat;Monkey
特异性The antibody detects endogenous level of total Vimentin protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.452~456(G-Q-V-I-N) derived from Human Vimentin.
基因/蛋白名称Vimentin
标记Unconjugated
别名CTRCT30
数据库入口号Swiss-Prot: P08670
NCBI Protein: NP_003371.2
Uniprot
P08670
计算分子量54kDa
实际分子量54-57kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000
Western blot analysis of extract from Hela, cos7 and JK cells using Vimentin Antibody.
Western Blot analysis of 22RV1, H460 and A549 cells using Vimentin Antibody.
Immunohistochemistry of paraffin-embedded Human colon tissue using Vimentin Antibody.
Immunohistochemistry of paraffin-embedded Mouse colon tissue using Vimentin Antibody.
Immunohistochemistry of paraffin-embedded Rat colon tissue using Vimentin Antibody.
Immunohistochemistry of paraffin-embedded Rat skin tissue using Vimentin Antibody.
Immunohistochemistry of paraffin-embedded Human lung tissue using Vimentin Antibody.
Immunohistochemistry of paraffin-embedded human breast cancer tissue using Vimentin Antibody.
Immunofluorescence staining of paraffin-embedded mesenchymal stem cells using Vimentin Antibody.
Immunofluorescence analysis of methanol-fixed Hela cells using Vimentin Antibody.
Immunofluorescence analysis of Rat skin using Vimentin Antibody.
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
Sommers C.L., Walker-Jones D., Heckford S.E., Worland P., Valverius E., Clark R., McCormick F.,
Cancer Res. 49:4258-4263(1989)
Kang S.-M., Shin M.-J., Kim J.-H., Oh J.-W. Proteomics 5:2227-2237(2005)
Ahmed B.A., Bukhari I.A., Jeffus B.C., Harney J.T., Thyparambil S., Ziu E., Fraer M., Rusch N.J. PLoS ONE 4:E4730-E4730(2009)
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