产品详情
产品名称Fibrillarin Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total Fibrillarin protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.297~301( L-T-L-E-P) derived from Human Fibrillarin
基因/蛋白名称Fibrillarin
标记Unconjugated
别名FBL; FIB; FLRN; RNU3IP1;
数据库入口号Swiss-Prot: P22087
NCBI Protein: NP_001427.2
Uniprot
P22087
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 37kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from 293, Hela cells using Fibrillarin Antibody #21495
Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.
Lischwe M.A., Ochs R.L., Reddy R., Cook R.G., Yeoman L.C., Tan E.M., Reichlin M., Busch H. J. Biol. Chem. 260:14304-14310(1985)
Yanagida M., Hayano T., Yamauchi Y., Shinkawa T., Natsume T., Isobe T., Takahashi N.J. Biol. Chem. 279:1607-1614(2004)
Wang Y., Liu J., Zhao H., Lue W., Zhao J., Yang L., Li N., Du X., Ke Y.Biochim. Biophys. Acta 1773:863-868(2007)
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et al,Synthetic Tryptanthrin Derivatives Induce Cell Cycle Arrest and Apoptosis via Akt and MAPKs in Human Hepatocellular Carcinoma Cells. In Biomedicines on 2021 Oct 24 by Jing-Yan Gao, Chih-Shiang Chang, et al..PMID: 34829756
, (2021),
PMID:
34829756
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