产品详情
产品名称ATR Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用IHC
种属反应性Human
特异性The antibody detects endogenous level of total ATR protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.426~430 (G-I-S-P-K) derived from Human ATR.
基因/蛋白名称ATR
标记Unconjugated
别名FRP1; MEC1; SCKL; SCKL1;
数据库入口号Swiss-Prot: Q13535
NCBI Protein: NP_001175.2
Uniprot
Q13535
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 250kd
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATR(Ab-428) Antiobdy #21505(left) or the same antibody preincubated with blocking peptide(right).
ATR encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist.
Zhou, X.Z. et al. (1999) Cell Mol. Life Sci. 56, 788-806.
Pinna, L.A. and Ruzzene, M. (1996) Biochim. Biophys. Acta 1314, 191-225.
Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.
Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-908.
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