产品详情
产品名称Paxillin Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IF
种属反应性Human
特异性The antibody detects endogenous level of total Paxillin protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.86~90 (P-V-Y-G-S) derived from Human Paxillin.
基因/蛋白名称Paxillin
标记Unconjugated
别名PAXI; PXN;
数据库入口号Swiss-Prot: P49023
NCBI Protein: NP_001074324.1
Uniprot
P49023
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:50~1:100
Western blot analysis of extracts from HUVEC cells using Paxillin(Ab-88) Antibody #21538 and the same antibody preincubated with blocking peptide.
Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(Ab-88) Antibody #21538.
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.
Bellis, S. L. et al. (1995) J. Biol. Chem. 270, 17437-17441
Schaller, M.D. and Parsons, J.T. (1995) Mol. Cell. Biol. 15, 2635-2645.
Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140.
如果您使用该产品21538发表了文章，请通知我们，让我们可以引用您的文献。
et al,Lipid rafts control human melanoma cell migration by regulating focal adhesion disassembly. In Biochim Biophys Acta on 2013 Dec by Ruifei Wang, Jiajia Bi, et al..PMID: 24055995
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24055995
et al,Lipid raft regulates the initial spreading of melanoma A375 cells by modulating 尾1 integrin clustering. In Int J Biochem Cell Biol on 2013 Aug by Ruifei Wang, Jiajia Bi,et
al..PMID: 23665237
, (2013),
PMID:
23665237
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