产品详情
产品名称syk Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC IF
种属反应性Human
特异性The antibody detects endogenous level of total syk protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa. 321~325 (N-P-Y-E-P) derived from Human syk.
基因/蛋白名称syk
标记Unconjugated
别名Spleen tyrosine kinase
数据库入口号Swiss-Prot: P43405
NCBI Protein: NP_001128524.1
Uniprot
P43405
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 72kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from JK cells using syk(Ab-323) Antibody #21546.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using syk(Ab-323) Antibody #21546(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using syk(Ab-323) Antibody #21546.
Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion
Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.
Turner, M. et al. (2000) Immunol. Today 21, 148-154.
Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874.
Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.
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