产品详情
产品名称PKM2 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC;IF;ELISA
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous levels of total PKM2 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.405~409(T-S-D-P-T) derived from Human PKM2.
基因/蛋白名称PKM2
标记Unconjugated
别名PKM; PK3; OIP3; PK2;
数据库入口号Swiss-Prot: P14618-1
NCBI Protein: NP_872270.1
Uniprot
P14618
计算分子量58kDa
实际分子量58-60kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-1:2000; IHC 1:50-1:200; IF 1:50-1:200; ELISA 1:5000-1:20000;
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PKM2 Antibody.
Immunohistochemistry analysis of paraffin-embedded Rat lung using PKM2 Antibody.
Immunohistochemistry analysis of paraffin-embedded Human colon using PKM2 Antibody.
Immunohistochemistry analysis of paraffin-embedded Human kidney using PKM2 Antibody.
Immunofluorescence staining of methanol-fixed MEF cells using PKM2 Antibody.Blue: DAPI for nuclear staining.
Immunofluorescence staining of methanol-fixed L929 cells using PKM2 Antibody.Blue: DAPI for nuclear staining.
Immunofluorescence staining of methanol-fixed U2OS cells using PKM2 Antibody.Blue: DAPI for nuclear staining.
Immunofluorescence staining of methanol-fixed HeLa cells using PKM2 Antibody.Blue: DAPI for nuclear staining.
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PKM antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: NIH3T3 Lane 3: C6 Predicted band size: 57kDa Observed band size: 57kDa
Western blot analysis of extracts from HepG2 and 293 cells using PKM2 Antibody.
Western blot analysis of extracts from various cell lines using PKM2 Antibody.
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Christofk, H.R. et al. (2008) Nature 452, 230-3.
Mazurek, S. et al. (2005) Semin Cancer Biol 15, 300-8.
Dombrauckas, J.D. et al. (2005) Biochemistry 44, 9417-29.
Hitosugi, T. et al. (2009) Sci Signal 2, ra73.
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et al,PKM2 Phosphorylates Histone H3 and Promotes Gene Transcription and Tumorigenesis. In Cell on 2012 Aug 17 by Weiwei Yang, Yan Xia, et al..PMID: 22901803
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et al,Nuclear PKM2 expression, an independent risk factor for ER after curative resection of hepatocellular carcinoma.In Biomed Pharmacother.On 2016 Dec by Fan F, Wu H et al..PMID: 27894667
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et al,PKM2 regulates chromosome segregation and mitosis progression of tumor cells.In Mol Cell on 2014 Jan 9 by Yuhui Jiang, Xinjian Li et al..PMID: 24316223
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et al,Relationship between 18-F-fluoro-deoxy-d-glucose uptake and expression of glucose transporter 1 and pyruvate kinase M2 in intrahepatic cholangiocarcinoma.In Dig Liver Dis
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et al,PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis.In Nat Commun on 2016 Aug 3 by Ji Liang , Ruixiu Cao et al..PMID: 27485204
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et al,PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells.In Nat Commun on 2014 Nov 21 by Yuhui Jiang , Yugang Wang et al..PMID: 25412762
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et al,Insulin regulates glucose consumption and lactate production through reactive oxygen species and pyruvate kinase M2. In Oxid Med Cell Longev on 2014 by Qi Li, Xue Liu, et al..PMID:24895527
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et al,Single-Cell FISH Analysis Reveals Distinct Shifts in PKM Isoform Populations during Drug Resistance Acquisition. In Biomolecules on 2022 Aug 6 by Seong Ho Kim, Ji Hun Wi, et al..PMID:36008976
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et al,Pyruvate Dehydrogenase A1 Phosphorylated by Insulin Associates with Pyruvate Kinase M2 and Induces LINC00273 through Histone Acetylation. In Biomedicines on 2022 May 27 by Abu Jubayer Hossain, Rokibul Islam, et al..PMID:35740278
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