产品详情
产品名称a-Tubulin Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total alpha-Tubulin protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.437-441(V-D-S-V-E) derived from Human alpha-Tubulin.
基因/蛋白名称a-Tubulin
标记Unconjugated
别名K-ALPHA-1
数据库入口号Swiss-Prot: P68363
NCBI Protein: NP_006073.2
Uniprot
P68363
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 52kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela and HT29 cells using alpha-Tubulin Antibody #21581.
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the a-chain.
Cowan N.J., Dobner P., Fuchs E.V., Cleveland D.W.Mol. Cell. Biol. 3:1738-1745(1983)
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