产品详情
产品名称CD209(DC-SIGN) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB
种属反应性Human
特异性The antibody detects endogenous level of total CD209 (DC-SIGN) protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.389~393( E-Q-F-L-S )derived from Human CD209 (DC-SIGN).
基因/蛋白名称CD209(DC-SIGN)
标记Unconjugated
别名CDSIGN; CLEC4L; DC-SIGN
数据库入口号Swiss-Prot: Q9NNX6
NCBI Protein: NP_066978.1
Uniprot
Q9NNX6
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 46kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from JK, HUVEC, HepG2 and Hela cells using CD209(DC-SIGN) Antibody #21600
Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.
Geijtenbeek T.B.H., Kwon D.S., Torensma R., van Vliet S.J. Cell 100:587-597(2000)
Geijtenbeek T.B.H., Krooshoop D.J., Bleijs D.A., van Vliet S.J. Nat. Immunol. 1:353-357(2000)
Engering A., Geijtenbeek T.B.H., van Vliet S.J., van Kooyk Y.J. Immunol. 168:2118-2126(2002)
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