产品详情
产品名称SPARC Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB
种属反应性Human
特异性The antibody detects endogenous level of total SPARC protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.52~56 (V-G-E-F-D) derived from Human SPARC.
基因/蛋白名称SPARC
标记Unconjugated
别名ON; BM-40;
数据库入口号Swiss-Prot: P09486
NCBI Protein: NP_003109.1
Uniprot
P09486
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 42kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from T98G cells using SPARC Antibody #21633
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
Kelm R.J. Jr., Mann K.G. Blood 75:1105-1113(1990)
Wewer U.M., Albrechtsen R., Fisher L.W. Am. J. Pathol. 132:345-355(1988)
Kuhn C., Mason R.J.Am. J. Pathol. 147:1759-1769(1995)
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