产品详情
产品名称Twist1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Affinity purification
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous levels of total Twist1 protein.
免疫原类型Peptide-KLH
免疫原描述Recombinant fusion protein of Twist (NP_000465.1).
基因/蛋白名称Twist1
标记Unconjugated
别名SCS; ACS3; CRS1; BPES2; BPES3
数据库入口号Swiss-Prot: Q15672
NCBI Protein: NP_000465.1
Uniprot
Q15672
计算分子量21kDa
配方PBS with 0.05% proclin300, 50% glycerol, pH7.3.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500 - 1:1000IHC 1:50 - 1:200;
Western blot analysis of various lysates, using Twist Rabbit pAb at 1:500 dilution.
Immunohistochemistry analysis of paraffin-embedded Rat brain using Twist Rabbit pAb at dilution of 1:200 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Twist Rabbit pAb at dilution of 1:200 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Mouse lung using Twist Rabbit pAb at dilution of 1:200 (40x lens).
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
"Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity."Sosic D., Richardson J.A., Yu K., Ornitz D.M., Olson E.N.Cell 112:169-180(2003)
"Mutations of the TWIST gene in the Saethre-Chotzen syndrome."el Ghouzzi V., le Merrer M., Perrin-Schmitt F., et al., Nat. Genet. 15:42-46(1997)
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et al,Metformin reverses stem cell?like HepG2 sphere formation and resistance to sorafenib by attenuating epithelial?mesenchymal transformation.In Mol Med Rep.On 2018 Oct by Feng Y, Guo X et al..PMID:30106125
, (2018),
PMID:
30106125
et al,Wogonoside Inhibits Invasion and Migration Through Suppressing TRAF2/4 Expression in Breast Cancer.In J Exp Clin Cancer Res on 2017 Aug 3 by Yuyuan Yao , Kai Zhao,et al..PMID: 28774312
, (2017),
PMID:
28774312
et al,Honokiol inhibits bladder cancer cell invasion through repressing SRC锟? expression and epithelial鈥憁esenchymal transition.In Oncol Lett on 2017 Oct by Lan Shen, Fang Zhang,et al..PMID: 28943942
, (2017),
PMID:
28943942
et al,SALL4, a novel marker for human gastric carcinogenesis and metastasis.In Oncogene on 2014 Nov 27 by L Zhang , Z Xu et al..PMID:24276240
, (2014),
PMID:
24276240
et al,TBBPA stimulated cell migration of endometrial cancer via the contribution of NOX-generated ROS in lieu of energy metabolism. In J Hazard Mater
on 2020 Dec 5 by Huilan Su, Ge Guan, et al..PMID:32569978
, (2020),
PMID:
32569978
et al,Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis. In Front Cell Dev Biol on 2020 Nov 25 by Guangying Cui, Huifen Wang, et al..PMID:33324633
, (2020),
PMID:
33324633
et al,The essential role of PRAK in tumor metastasis and its therapeutic potential. In Nat Commun on 2021 Mar 19 by Yuqing Wang, Wei Wang, et al..PMID:33741957
, (2021),
PMID:
33741957
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et al,Interleukin-6 and Hypoxia Synergistically Promote EMT-Mediated Invasion in Epithelial Ovarian Cancer via the IL-6/STAT3/HIF-1 α Feedback LoopInAnal Cell PatholOn2023 Feb 13byTongshuo Zhang?1?2?3,?Jing Yang et al..PMID:?36814597
, (2023),
PMID: 36814597
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