产品详情
产品名称SCF Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB
种属反应性Human;Mouse
特异性The antibody detects endogenous level of total SCF protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.265~269(E-K-E-R-E) derived from Human SCF.
基因/蛋白名称SCF
标记Unconjugated
别名SF; MGF; FPH2; KL-1; Kitl
数据库入口号Swiss-Prot: P21583
NCBI Protein: NP_000890.1
Uniprot
P21583
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 25-38kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from A549, A431, Hela and 3T3 cells using SCF Antibody #21670.
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol-1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Lu H.S., Clogston C.L., Wypych J. Arch. Biochem. Biophys. 298:150-158(1992)
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et al,Effect of lipopolysaccharide stimulation on stem cell-associated marker-expressing cells. In Int Endod J.On 2018 Feb by Sueyama Y, Kaneko T et al..PMID: 27977848
, (2018),
PMID:
27977848
et al,Effect of lipopolysaccharide stimulation on stem cell-associated marker-expressing cells.In Int Endod J. On 2018 Feb by Sueyama Y, Kaneko T et al..PMID:27977848
, (2018),
PMID:
27977848
et al,Effect of Lipopolysaccharide Stimulation on Stem Cell-Associated Marker-Expressing Cells.In Int Endod J on 2018 Feb by Y Sueyama , T Kaneko,et al..PMID: 27977848
, (2017),
PMID:
27977848
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