产品详情
产品名称Histone H3 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC;IF;IP;ELISA
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total Histone H3 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.26-30(A-R-K-S-A) derived from Human Histone H3.
基因/蛋白名称Histone H3
标记Unconjugated
别名H3/A; H3FA
数据库入口号Swiss-Prot: P68431
NCBI Gene ID: 8350
NCBI mRNA: NM_003529.2
NCBI Protein: NP_003520.1
Uniprot
P68431
计算分子量17kDa
实际分子量17kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C
应用详情
WB 1:1000-1:5000; IHC 1:50-1:200; IF 1:50-1:200; ELISA 1:5000-1:20000; IP 1:50-1:200;
Western blot analysis of extracts from HT29 and C6 cells using Histone H3 Antibody.
Western blot analysis of extracts from MCF, Hela cells and Mouse lung using Histone H3 Antibody.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Histone H3 Antibody.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Histone H3 Antibody.
Immunohistochemical analysis of paraffin-embedded Human liver tissue using Histone H3 Antibody.
Immunohistochemical analysis of paraffin-embedded Human brain tissue using Histone H3 Antibody.
Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using Histone H3 Antibody.
Immunofluorescence staining of methanol-fixed Hela cells using Histone H3 Antibody.
Immunofluorescence staining of methanol-fixed Rat liver using Histone H3 Antibody.
Immunofluorescence staining of methanol-fixed MCF-7 cells using Histone H3 Antibody(green). The nuclear counter stain is DAPI (blue).
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Choi H.S., Choi B.Y., Cho Y.-Y., Zhu F., Bode A.M., Dong Z.J. Biol. Chem. 280:13545-13553(2005)
Tan M., Luo H., Lee S., Jin F., Yang J.S., Montellier E., Buchou T., Cheng Z., Rousseaux S., Rajagopal N., Lu Z., Ye Z., Zhu Q., Wysocka J., Ye Y., Khochbin S., Ren B., Zhao Y.
Cell 146:1016-1028(2011)
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