产品详情
产品名称AKAP12 antibody
来源种属Rabbit
克隆性Polyclonal
纯化Purified by antigen-affinity chromatography.
应用WB IHC IF
种属反应性Human
免疫原类型Peptide
免疫原描述Synthetic peptide contain a sequence corresponding to a region within amino acids 1719 and 1782 of AKAP12
基因/蛋白名称AKAP12
标记Unconjugated
数据库入口号NCBI Gene ID: 9590
NCBI mRNA#: NM_005100
NCBI Protein#: NP_005091
浓度0.7mg/ml
配方Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
保存Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
应用详情
Predicted MW: 192kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Sample (30ug whole cell lysate)
A: HeLa S3
5% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded serous OVCA, using AKAP12 antibody at 1: 100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKAP12 antibody at 1: 200 dilution.
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]
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