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位置: 首页 > 多克隆抗体 > APRT antibody

APRT antibody#22026

是否有货: 有货
产品详情

产品名称APRT antibody

来源种属Rabbit

克隆性Polyclonal

纯化Purified by antigen-affinity chromatography.

应用WB IHC IF

种属反应性Human

免疫原类型Recombinant protein

免疫原描述Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 104 of APRT

基因/蛋白名称APRT

标记Unconjugated

数据库入口号NCBI Gene ID: 353
NCBI mRNA#: NM_001030018
NCBI Protein#: NP_001025189

浓度1mg/ml

配方Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

保存Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

应用详情

Predicted MW: 15kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:500
Immunofluorescence: 1:100-1:200
 

Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using APRT antibody at 1: 500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using APRT antibody at 1: 200 dilution.

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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