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alpha Tubulin antibody#22038

是否有货: 有货
产品详情

产品名称alpha Tubulin antibody

来源种属Rabbit

克隆性Polyclonal

纯化Purified by antigen-affinity chromatography.

应用WB IHC IF

种属反应性Human

免疫原类型Recombinant protein

免疫原描述Recombinant protein fragment contain a sequence corresponding to a region within amino acids 227 and 450 of alpha Tubulin

基因/蛋白名称alpha Tubulin

标记Unconjugated

数据库入口号NCBI Gene ID: 7846
NCBI mRNA#: BC006468
NCBI Protein#: AAH06468

浓度0.2mg/ml

配方Supplied in 0.1M Tris-buffered saline with 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

保存Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

应用详情

Predicted MW: 50kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: JurKat
D: Raji
10% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded Breast ca, using alpha Tubulin 1A antibody at 1: 250 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Tubulin 1A antibody at 1: 200 dilution.

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. [provided by RefSeq]

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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