产品详情
产品名称MAF1 antibody
来源种属Rabbit
克隆性Polyclonal
纯化Purified by antigen-affinity chromatography.
应用WB IF
种属反应性Human
免疫原类型Recombinant protein
免疫原描述Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 171 of MAF1
基因/蛋白名称MAF1
标记Unconjugated
数据库入口号NCBI Gene ID: 84232
NCBI mRNA#: NM_032272
NCBI Protein#: NP_115648
浓度1mg/ml
配方Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
保存Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
应用详情
Predicted MW: 29kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200
Sample(30 ug whole cell lysate)
A: A431
B: MOLT4
12% SDS PAGE
Primary antibody diluted at 1: 1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAF1 antibody at 1: 200 dilution.
This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq]
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