产品详情
产品名称Strep-tag II Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Affinity chromatography purified via peptide column
应用ELISA WB
免疫原类型Peptide
免疫原描述Raised against the amino acid sequence ¡±srWSHPQFEKsh¡±.
基因/蛋白名称Strep-tag II
标记Unconjugated
别名StrepII-tag
浓度1mg/ml
配方Supplied in PBS containing 0.02% sodium azide.
保存Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
应用详情
Western blot analysis of (A) 5 and (B) 25ng of StrepII-tagged recombinant protein with StrepII antibody at 1 ug/mL.
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins.
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