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Placental alkaline phosphatase (PLAP) Monoclonal Antibody#27192

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产品详情

产品名称Placental alkaline phosphatase (PLAP) Monoclonal Antibody

克隆号8F10-2C10-C9

来源种属Mouse

克隆性Monoclonal

亚型IgG2b

纯化Affinity purified

应用WB ICC

种属反应性Human

特异性This antibody detects endogenous levels of ALPP and does not cross-react with related proteins.

免疫原类型Recombinant Protein

免疫原描述Purified recombinant human ALPP protein fragments expressed in E.coli.

基因/蛋白名称Placental alkaline phosphatase(PLAP)

标记Unconjugated

别名Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1;

数据库入口号Uniprot: P05187

Gene ID: 250

Uniprot P05187

实际分子量76kd

配方Purified mouse monoclonal in PBS(pH 7.4) containing with 0.2% sodium azide, 50% glycerol, 0.1%BSA

保存store at -20˚C

应用详情
Western blotting: 1:500
Immunocytochemistry: 1:300
Immunocytochemistry stain of Hela using ALPP antibody (1:300).
Western blot detection of ALPP in Hela,LNCap,human Placenta &A549 cell lysates and using ALPP antibody (1:500 diluted). Predicted band size: 76KDa Observed band size:76KDa

 There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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