产品详情
产品名称LC3B Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IHC,IF
种属反应性Human;Mouse;Rat
免疫原描述A synthetic peptide of human LC3B (NP_073729.1).
标记Unconjugated
别名LC3B;ATG8F;MAP1LC3B-a;MAP1A/1BLC3;MAP1LC3B;LC3A/LC3B
数据库入口号GeneID:81631
Swiss Prot:Q9GZQ8
Uniprot
Q9GZQ8
计算分子量14kDa
实际分子量14kDa/15kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 173% glycerol.
保存Store at -20˚C. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.126.
应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Immunofluorescence analysis of LC3B knockout 293T cells treated by chloroquine and wild-type 293T cells ��wild-type 293T cells treated by chloroquine using LC3B Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse brain using LC3B antibody at dilution of 1:100 .
Immunohistochemistry of paraffin-embedded rat brain using LC3B antibody at dilution of 1:100 .
Western blot analysis of extracts of 293T cells, using LC3B antibody at 1:1000 dilution.293T cells were treated by Chloroquine at 37�� for 20 hours.
Western blot analysis of extracts of various cell lines, using LC3B antibody at 1:1000 dilution.
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
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et al,The AMPK-dependent inhibition of autophagy plays a crucial role in protecting photoreceptor from photooxidative injury In J Photochem Photobiol B On2023 AugbyYu-Lin Li , Tian-Zi Zhang et al..PMID:37302163
, (2023),
PMID:
37302156
et al,Cannabinoid receptor-2 attenuates neuroinflammation by promoting autophagy-mediated degradation of the NLRP3 inflammasome post spinal cord injury
, (2022),
PMID:
36238284
Yes
