产品详情
产品名称NLRP3 Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IHC,IF
种属反应性Human;Mouse;Rat
免疫原描述A synthetic peptide of human NLRP3 (NP_001120934.1).
标记Unconjugated
别名NLRP3;AGTAVPRL;AII;AVP;C1orf7;CIAS1;CLR1.1;FCAS;FCAS1;FCU;MWS;NALP3;PYPAF1
数据库入口号GeneID:114548
Swiss Prot:Q96P20
Uniprot
Q96P20
计算分子量83kDa/105kDa/111kDa/112kDa/115kDa/118kDa
实际分子量118kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 239% glycerol.
保存Store at -20˚C. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.192.
应用详情
WB 1:1000 - 1:3000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Immunofluorescence analysis of Raw264.7 cells using NLRP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Raw264.7 cells using NLRP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat lung cells using NLRP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat lung using NLRP3 antibody at dilution of 1:100 .
Immunohistochemistry of paraffin-embedded human appendix using NLRP3 antibody at dilution of 1:100 .
Immunohistochemistry of paraffin-embedded mouse spleen using NLRP3 antibody at dilution of 1:100 .
Western blot analysis of extracts of U-937 cells, using NLRP3 antibody at 1:1000 dilution.
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.
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et al,The Extracts of Angelica sinensis and Cinnamomum cassia from Oriental Medicinal Foods Regulate Inflammatory and Autophagic Pathways against Neural Injury after Ischemic Stroke. In Oxid Med Cell Longev on 2021 Jun 26 by Cheng Luo, Qi Chen,et al..PMID:34257822
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et al,Alpha-lipoic acid alleviates NAFLD and triglyceride accumulation in liver via modulating hepatic NLRP3 inflammasome activation pathway in type 2 diabetic rats. In Food Sci Nutr on 2021 Mar 13 by Chih-Yuan Ko, Yangming Martin Lo, et al..PMID: 34026086
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et al,Histone acetylation regulates ORMDL3 expression‐mediated NLRP3 inflammasome overexpression during RSV‐allergic exacerbation miceInJ Cell PhysiolOn2023 DecbyQi Cheng 1, Fanghan He et al..PMID: 37877592
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et al,Alleviation of Splenic Injury by CB001 after Low-Dose Irradiation Mediated by NLRP3/Caspase-1-BAX/Caspase-3 AxisInRadiat ResOn2023 Dec 29byChangkun Hu?1?2,?Zebin Liao et al..PMID:?38154483
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et al,Dopamine Activates the D1R-Zn2+ Signaling Pathway to Trigger Inflammatory Response in Primary-Cultured Rat Embryonic Cortical NeuronsInCell Mol NeurobiolOn2023 OctbyHui-Chiun Tseng 1, Chien-Yuan Pan et al..PMID: 37289255
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et al,Neuritin attenuates neuroinflammation and apoptosis in early brain injury after subarachnoid hemorrhage via endoplasmic reticulum stress-related inflammatory pathways. In Brain Res on 2024 Dec 15 by Kunhao Ren, Linzhi Dai,et al..PMID:39454807
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et al,NLRP3 promotes radiation‐induced brain injury by regulating microglial pyroptosis. In Neuropathol Appl Neurobiol on 2024 Jun
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et al,Glutamate Gradually Elevates [Zn2+] i via the CaM− CaMKII− NOS Cascade in Primary Cultured Rat Embryonic Cortical Neurons
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PMID:
Yes
