产品详情
产品名称PINK1 Rabbit Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB;IHC;IF
种属反应性Human;Mouse;Rat
免疫原描述Recombinant fusion protein of human PINK1 (NP_115785.1).
标记Unconjugated
别名PINK1;BRPK;PARK6
数据库入口号Swiss Prot:Q9BXM7
GeneID:65018
Uniprot
Q9BXM7
计算分子量63kDa
实际分子量60kDa
配方PBS with 0.09% sodium azide,0.05% BSA,50% glycerol,pH7.3.
保存Store at -20˚C. Avoid freeze / thaw cycles.
应用详情
WB 1:1000 - 1:5000
IHC 1:200 - 1:800
IF 1:50 - 1:200
Western blot analysis of lysates from Rat skeletal muscle, using PINK1 Rabbit Polyclonal Antibody at 1:2000 dilution.;
Secondary antibody: Goat anti-Rabbit IgG at 1:10000 dilution.;
Lysates/proteins: 25ug per lane.;
Blocking buffer: 3% nonfat dry milk in TBST.;
Exposure time: 90s.
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma tissue using PINK1 Rabbit Polyclonal Antibody at a dilution of 1:400 (40x lens).;
High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffinembedded Human breast cancer tissue using PINK1 Rabbit Polyclonal Antibody at a dilution of 1:400 (40 lens).;
High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffinembedded Human kidney tissue using PINK1 Rabbit Polyclonal Antibody at a dilution of 1:400(40 lens).;
High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.
Immunofluorescence analysis of NIH-3T3 cells using PINK1 Rabbit Polyclonal Antibody at dilution of 100 (40 lens).;
Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG at 1:500 dilution.;
Blue:DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using PINK1 Rabbit Polyclonal Antibody at dilution of 100 (40 lens).;
Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG at 1:500 dilution.;
Blue: DAPI fornuclear staining.
This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It isthought to protect cells from stress-induced mitochondrial dysfunction. Mutations in thisgene cause one form of autosomal recessive early-onset Parkinson disease.
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