产品详情
产品名称CALR Antibody
来源种属Rabbit
克隆性Polyclonal
应用ELISA WB IHC
种属反应性Human;Mouse
特异性The antibody detects endogenous level of total CALR protein.
免疫原类型Recombinant protein
免疫原描述Fusion protein corresponding to a region derived from 20-218 amino acids of human calreticulin
基因/蛋白名称CALR
标记Unconjugated
别名Calreticulin, RO; CRT; SSA; cC1qR
数据库入口号Genbank No.: BC002500
Uniprot
BC002500
配方Supplied at 1mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
保存Store at -20˚C/1 year
应用详情
Predicted MW: 48kd
ELISA: 1:2000-1:10000
Western blotting: 1:1000-1:5000
Immunohistochemistry: 1:50-1:200
Gel: 10%SDS-PAGE
Lane1: Hela cell lysate
Lane2: 293T cell lysate
Lane3: NIH/3T3 cell lysate
Lysates: 40 ug per lane
Primary antibody: 1/500 dilution
Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Exposure time: 30 seconds
The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using 31077 (CALR Antibody) at dilution 1/30, on the right is treated with the fusion protein.
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element.
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et al,Autoantibody against Tumor-Associated Antigens as Diagnostic Biomarkers in Hispanic Patients with Hepatocellular Carcinoma. In Cells on 2022 Oct 14 by Yangcheng Ma, Cuipeng Qiu, et al..PMID:36291095
, (2022),
PMID:
36291095
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