产品详情
产品名称MYL9 Antibody
来源种属Rabbit
克隆性Polyclonal
应用ELISA WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total MYL9 protein.
免疫原类型Peptide
免疫原描述Synthetic peptide corresponding to a region derived from 2-14 amino acids of Human myosin, light chain 9, regulatory
基因/蛋白名称MYL9
标记Unconjugated
别名myosin, light chain 9, regulatory, LC20, MLC2, MRLC1, MYRL2, MLC-2C
数据库入口号Genbank No.: NP_006088
Uniprot
NP_006088
配方Supplied at 3mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
保存Store at -20˚C/1 year
应用详情
Predicted MW: 14kd
ELISA: 1:2000-1:10000
Western blotting: 1:1000-1:5000
Immunohistochemistry: 1:50-1:200
Gel: 12%SDS-PAGE
Lane1: Jurkat cell lysate
Lane2: 231 cell lysate
Lysates: 40 ug per lane
Primary antibody: 1/1500 dilution
Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Exposure time: 30 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 31244(MYL9 Antibody) at dilution 1/80, on the right is treated with the synthetic peptide.
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
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et al,Identification and validation of critical genes with prognostic value in gastric cancer
, (2022),
PMID:
36589754
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