产品详情
产品名称NPHS1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was purified by immunogen affinity chromatography.
应用WB;IHC;ICC/IF
种属反应性Human;Mouse
特异性The antibody detects endogenous level of total NPHS1 protein.
免疫原类型Peptide
免疫原描述KLH-conjugated synthetic peptide of human Nephrin.
基因/蛋白名称NPHS1
标记Unconjugated
别名nephrosis 1, congenital, Finnish type (nephrin), CNF, NPHN, nephrin
数据库入口号Genbank No.: NP_004637
Uniprot
NP_004637
浓度1 mg/ml
配方Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
保存Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
应用详情
WB 1:500-1:1000;
IHC 1:50-1:100;
ICC/IF 1:50-1:200;
Western blot analysis of Nephrin expression in HeLa (A), HEK293T (B), PC12 (C) whole cell lysates.
(Predicted band size:130; 134 kDa; Observed band size: 130 kDa)
Immunohistochemical analysis of Nephrin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Nephrin staining in mouse kidney. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.In Western blots, nephrin antibodies generated against the two terminal extracellular Ig domains of recombinant human nephrin recognized a 180-kDa protein in lysates of human glomeruli and a 150-kDa protein in transfected COS-7 cell lysates.
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et al,Effects of transcription factor EB on oxidative stress and apoptosis Induced by high glucose In podocytes. In Int J Mol Med on 2019 May 24 by Kang Y, Li Y, et al.. PMID: 31173156
, (2019),
PMID:
31173156
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