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位置: 首页 > 多克隆抗体 > PDK4 Antibody

PDK4 Antibody#31258

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产品详情

产品名称PDK4 Antibody

来源种属Rabbit

克隆性Polyclonal

应用ELISA WB IHC

种属反应性Human;Mouse;Rat

特异性The antibody detects endogenous level of total PDK4 protein.

免疫原类型Peptide-KLH

免疫原描述Synthetic peptide corresponding to a region derived from 101-113 amino acids of Human pyruvate dehydrogenase kinase, isozyme 4

基因/蛋白名称PDK4

标记Unconjugated

别名Pyruvate dehydrogenase kinase, isozyme 4

数据库入口号Genbank No.: NP_002603

Uniprot NP_002603

配方Supplied at 1.6mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Predicted MW: 46kd
ELISA: 1:1000-1:5000
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200

Gel: 10%SDS-PAGE
Lysate: 60 µg Mouse pancreas tissue lysate
Primary antibody: 1/700 dilution
Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Exposure time: 2 minutes
The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using 31258 (PDK4 Antibody) at dilution 1/50, on the right is treated with the synthetic peptide.
Immunohistochemistry of paraffin-embedded Human tonsil tissue using PDK4 antibody at dilution of 1:50

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.

如果您使用该产品31258发表了文章,请通知我们,让我们可以引用您的文献。

et al,Targeting Lactate Dehydrogenase A with Catechin Resensitizes SNU620/5FU Gastric Cancer Cells to 5-Fluorouracil. In Int J Mol Sci on 2021 May 20 by Jung Ho Han, MinJeong Kim,et al..PMID:34065602 , (2021),
PMID: 34065602

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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