产品详情
产品名称RNF126 Antibody
来源种属Rabbit
克隆性Polyclonal
应用ELISA WB IHC
种属反应性Human
特异性The antibody detects endogenous level of total RNF126 protein.
免疫原类型Peptide
免疫原描述Synthetic peptide corresponding to a region derived from 106-119 amino acids of Human ring finger protein 126
基因/蛋白名称RNF126
标记Unconjugated
别名Ring finger protein 126
数据库入口号Genbank No.: NP_919442
Uniprot
NP_919442
浓度0.3mg/ml
配方Supplied at 0.7mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
保存Store at -20˚C/1 year
应用详情
Predicted MW: 38kd
ELISA: 1:1000-1:5000
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:25-1:100
Gel: 10%SDS-PAGE
Lane1: Jurkat cell lysate
Lane2: K562 cell lysate
Lysates: 40 ug per lane
Primary antibody: 1/350 dilution
Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Exposure time: 20 seconds
The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using 31265 (RNF126 Antibody) at dilution 1/20, on the right is treated with the synthetic peptide.
The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. RNF126 interacts with p21 and RNF126 over-expression increased p21 protein ubiquitination in an E3 ligase activity-dependent manner. RNF126 knockdown induced loss of cell viability in MDA-MB-231 and PC-3 can be partially rescued by depletion of p21. RNF126 stable knockdown in PC3 inhibited tumor growth in SCID mice.
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et al,CHFR-mediated degradation of RNF126 confers sensitivity to PARP inhibitors in triple-negative breast cancer cells. In Biochem Biophys Res Commun on 2021 Oct 8 by Wenjing Wu, Jianli Zhao, et al..PMID:34388456
, (2021),
PMID:
34388456
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