产品详情
产品名称NIT2 Antibody
来源种属Rabbit
克隆性Polyclonal
应用ELISA WB IHC
种属反应性Human;Mouse
特异性The antibody detects endogenous level of total NIT2 protein.
免疫原类型Peptide
免疫原描述Synthetic peptide corresponding to a region derived from 264-276 amino acids of Human itrilase family, member 2
基因/蛋白名称NIT2
标记Unconjugated
别名Itrilase family, member 2
数据库入口号Genbank No.: NP_064587
Uniprot
NP_064587
浓度0.4mg/ml
配方Supplied at 1.4mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
保存Store at -20˚C/1 year
应用详情
Predicted MW: 31kd
ELISA: 1:2000-1:10000
Western blotting: 1:1000-1:5000
Immunohistochemistry: 1:50-1:200
Gel: 10%SDS-PAGE
Lane1: Mouse liver tissue lysate
Lane2: Mouse kidney tissue lysate
Lane3: RAW264.7 cell lysate
Lysates: 40 ug per lane
Primary antibody: 1/950 dilution
Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Exposure time: 90 seconds seconds
The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using 31280 (NIT2 Antibody) at dilution 1/40, on the right is treated with the synthetic peptide.
Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.
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et al, Downregulation of NIT2 inhibits colon cancer cell proliferation and induces cell cycle arrest through the caspase-3 and PARP pathways.In Int J Mol Med on 2015 May by Bo'an Zheng , Rui Chai et al..PMID:25738796
, (2015),
PMID:
25738796
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