产品详情
产品名称PARP1 Antibody
来源种属Rabbit
克隆性Polyclonal
应用ELISA WB IHC
种属反应性Human
特异性The antibody detects endogenous level of total PARP1 protein.
免疫原类型Peptide
免疫原描述Synthetic peptide corresponding to a region derived from 12-21 amino acids of human poly (ADP-ribose) polymerase 1
基因/蛋白名称PARP1
标记Unconjugated
别名poly (ADP-ribose) polymerase 1, PARP, PPOL, ADPRT, ARTD1, ADPRT1, PARP-1, ADPRT 1, pADPRT-1
数据库入口号Genbank No.: NP_001609.2
Uniprot
NP_001609.2
配方Supplied at 0.7mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
保存Store at -20˚C/1 year
应用详情
Predicted MW: 113kd
ELISA: 1:2000-1:5000
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
Gel: 8%SDS-PAGE
Lane1: Hela cell lysate
Lane2: K562 cell lysate
Lane3: Jurkat cell lysate
Lysates: 40 ug per lane
Primary antibody: 1/350 dilution
Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Exposure time: 1 second
The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using 31288 (PARP1 Antibody) at dilution 1/40, on the right is treated with the synthetic peptide.
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
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