产品详情
产品名称BAK1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB,IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total BAK1 protein.
免疫原类型Peptide
免疫原描述A synthetic peptide of human BAK1.
基因/蛋白名称BAK1
标记Unconjugated
别名BAK1; BAK; BAK-LIKE; BCL2L7; CDN1
数据库入口号Swiss-Prot:Q16611
NCBI Gene ID:578
Uniprot
Q16611
实际分子量23KD
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB 1:500 - 1:2000
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using BAK1 antibody at 1:1000 dilution.
Immunofluorescence analysis of HeLa cells using BAK1 antibody. Blue: DAPI for nuclear staining.
Bak is a proapoptotic member of the Bcl-2 family (1). This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway (2,3). Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death (4,5).
如果您使用该产品32009发表了文章，请通知我们，让我们可以引用您的文献。
有货
