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Western blot analysis of lysates from various cell lines using BCL2 Antibody. Lane1: mouse liver Lane2: HepG2 Lane3: JK Lane 4: MCF-7 Lane5: Hela
(Green)primary antibody was diluted at 1:1000, 4℃ over night, Dylight 800 secondary antibody was diluted at 1:10000, 37℃ 1hour. (Red) Actin β Monoclonal Antibody was diluted at 1:5000 as loading control, 4℃ over night,Dylight 680 secondary antibody was diluted at 1:10000, 37℃ 1hour.
(Green)primary antibody was diluted at 1:1000, 4℃ over night, Dylight 800 secondary antibody was diluted at 1:10000, 37℃ 1hour. (Red) Actin β Monoclonal Antibody was diluted at 1:5000 as loading control, 4℃ over night,Dylight 680 secondary antibody was diluted at 1:10000, 37℃ 1hour.
Western blot analysis of lysates from various cell lines using BCL2 Antibody. Lane1: 3T3 Lane2: mouse brain Lane3: Mouse lung Lane 4: MCF-7 Lane5: Hela Lane6: 293T Lane7: Mouse Brain Lane8: Mouse Spleen Lane9: Rat Kidney Lane10: Rat Liver
Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution.
Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using BCL2 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded rat pancreas tissue using BCL2 Antibody.
Immunohistochemistry analysis of paraffin-embedded rat brain tissue using BCL2 Antibody.
Immunohistochemistry of paraffin-embedded human breast cancer using BCL2 Antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of methanol-fixed HepG2 cells using BCL2 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of methanol-fixed NIH/3T3 cells using BCL2 Antibody.
Immunofluorescence analysis of methanol-fixed C6 cells using BCL2 Antibody.
Immunofluorescence analysis of L929 cells using BCL2 Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
