产品详情
产品名称BID Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB,IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total BID protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant protein of human BID.
基因/蛋白名称BID
标记Unconjugated
别名BID; FP497; MGC15319; MGC42355;
数据库入口号Swiss-Prot:P55957
NCBI Gene ID:637
Uniprot
P55957
实际分子量22KD
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB 1:500 - 1:2000
IF 1:50 - 1:200
Western blot analysis of extracts of HL-60 cells, using BID antibody.
Immunofluorescence analysis of C6 cells using BID antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using BID antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using BID antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
The BH3 domain-only protein, BID, a death agonist member of the Bcl-2/Bcl-xL family (1), is localized in the cytosolic fraction of cells as an inactive precursor (2,3). Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved BID translocates to mitochondria and induces cytochrome c release and mitochondrial damage (2-5). Thus, BID relays an apoptotic signal from the cell surface to mitochondria. However, the precise molecular mechanism for the translocation of the cleaved BID, and for the subsequent release of cytochrome c during apoptosis, is still unclear.
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et al,Magnesium-Assisted Cisplatin Inhibits Bladder Cancer Cell Survival by Modulating Wnt/β-Catenin Signaling Pathway. In Front Pharmacol on 2022
Jan 27 by Tianye Li, Zihan Tang,et al..PMID:35153759
, (2022),
PMID:
35153759
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