产品详情
产品名称IL1B Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB,IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total IL1B protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant protein of human IL1B.
基因/蛋白名称IL1B
标记Unconjugated
别名IL1B; IL-1; IL1-BETA; IL1F2; IL1beta
数据库入口号Swiss-Prot:P01584
NCBI Gene ID:3553
Uniprot
P01584
实际分子量31KD
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB 1:500 - 1:2000
IF 1:50 - 1:200
Western blot analysis of extracts of mouse liver, using IL1 beta antibody at 1:1000 dilution.
Immunofluorescence analysis of THP-1 cells using IL1 beta Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse lung using IL1 beta Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. PA700, PA28, and PA200 are three major protein Interleukin-1beta (IL-1beta), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1beta is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1beta is a good indicator of caspase-1 activity.
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et al,Targeting the AKT-P53/CREB pathway with epicatechin for improved prognosis of traumatic brain injury
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et al,Poly-(ADP-ribose) polymerases inhibition by olaparib attenuates activities of the NLRP3 inflammasome and of NF-κB in THP-1 monocytes
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et al,The role of FTO in m6A RNA methylation and immune regulation in Staphylococcus aureus infection-related osteomyelitis.
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39980685
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