产品详情
产品名称EMD Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB,IHC,IF
种属反应性Human;Mouse
特异性The antibody detects endogenous level of total EMD protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant protein of human EMD.
基因/蛋白名称EMD
标记Unconjugated
别名EMD; EDMD; LEMD5; STA;
数据库入口号Swiss-Prot:P50402
NCBI Gene ID:2010
Uniprot
P50402
实际分子量29KD
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
Immunofluorescence analysis of HeLa cells using EMD antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using EMD antibody at 1:1000 dilution.
Confocal immunofluorescence analysis of U-2 OS cells using EMD Polyclonal antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human uterus using EMD antibody at dilution of 1:100 (40x lens).
Emerin is a broadly expressed integral protein of the nuclear inner membrane (1). It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to-autointegration factor) and A- and B-type lamins, which are important in nuclear functions (2-5). Emerin may regulate gene expression through binding to other transcriptional regulators (6,7). Emerin binds to β-catenin and inhibits its nuclear accumulation (8). Recent studies demonstrate that Emerin is required for HIV-1 infectivity (9). Mutations in Emerin are a major cause of Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10).
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et al,Portulaca oleracea L. extract reduces hyperglycemia via PI3k/Akt and AMPK pathways in the skeletal muscles of C57BL/Ksj-db/db mice. In J Ethnopharmacol on 2020 Oct 5 by Ji Hyun Lee, Jae Eun Park,et al..PMID:32416244
, (2020),
PMID:
32416244
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