产品详情
产品名称CAPN1 Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IHC,IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total CAPN1 protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant fusion protein of human Calpain 1 (NP_005177.2).
基因/蛋白名称CAPN1
标记Unconjugated
别名CAPN1;CANP;CANP1;CANPL1;SPG76;muCANP;muCL;calpain 1
数据库入口号Uniprot:P07384
GeneID:823
Uniprot
P07384
Gene ID
823
实际分子量75-80KDa
浓度1.0mg/ml
配方PBS with 0.02% sodium azide,50% glycerol,pH7.3.
保存Store at -20˚C. Avoid freeze / thaw cycles.
应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Calpain 1 antibody.
Immunohistochemistry of paraffin-embedded rat brain using Calpain 1 Rabbit pAb.
Immunofluorescence analysis of HeLa cells using Calpain 1 antibody.
Immunofluorescence analysis of NIH/3T3 cells using Calpain 1 antibody.
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
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et al,Calcium efflux from the endoplasmic reticulum regulates cisplatin-induced apoptosis in human cervical cancer HeLa cells.In Oncol Lett.On 2016 Apr by Shen L, Wen N et al..PMID: 27073489
, (2016),
PMID:
27073489