产品详情
产品名称CREB1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB;IHC;IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total CREB1 protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant protein of human CREB1.
基因/蛋白名称CREB1
标记Unconjugated
别名CREB1; CREB; MGC9284;
数据库入口号Swiss-Prot:P16220
NCBI Gene ID:1385
Uniprot
P16220
计算分子量35kDa
实际分子量43kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Western blot analysis of extracts from various cell lines using CREB1 antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB Antibody (left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemistry analysis of paraffin-embedded Rat lung using CREB1 Antibody at dilution of 1:50 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Mouse brain using CREB1 Antibody at dilution of 1:50 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using CREB1 antibody.
Immunofluorescence analysis of HeLa cells using CREB1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of MCF-7 cells using CREB1 antibody . Blue: DAPI for nuclear staining.
Immunofluorescent analysis of CREB staining in BV2 cells.
CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).
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