产品详情
产品名称GPX4 Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IHC,IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total GPX4 protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant fusion protein of human GPX4 (NP_002076.2).
基因/蛋白名称GPX4
标记Unconjugated
别名GPX4;GPx-4;GSHPx-4;MCSP;PHGPx;SMDS;snGPx;snPHGPx
数据库入口号Uniprot:P36969
GeneID:2879
Uniprot
P36969
Gene ID
2879
实际分子量22KDa
浓度1.0mg/ml
配方PBS with 0.02% sodium azide,50% glycerol,pH7.3.
保存Store at -20˚C. Avoid freeze / thaw cycles.
应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using GPX4 antibody.
Immunohistochemistry of paraffin-embedded rat testis using GPX4 Rabbit pAb.
Immunohistochemistry of paraffin-embedded human appendix using GPX4 Rabbit pAb.
Immunohistochemistry of paraffin-embedded mouse testis using GPX4 Rabbit pAb.
Immunofluorescence analysis of C6 cells using GPX4 Rabbit pAb.
Immunofluorescence analysis of L929 cells using GPX4 Rabbit pAb.
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.
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