产品详情
产品名称Histone H3 Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB;IHC;IF;IP;ELSIA
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total Histone H3 protein.
免疫原类型Recombinant Protein
免疫原描述A synthetic peptide of human HIST3H3 (NP_003484.1).
基因/蛋白名称Histone H3
标记Unconjugated
别名H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A
数据库入口号Uniprot:Q16695
GeneID:8290
Uniprot
Q16695
Gene ID
8290
计算分子量16kDa
实际分子量17kDa
浓度1.0mg/ml
配方PBS with 0.02% sodium azide,50% glycerol,pH7.3.
保存Store at -20˚C. Avoid freeze / thaw cycles.
应用详情
WB 1:2000-1:10000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200;
Western blot analysis of extracts from various cell lines using Histone H3 antibody.
Western blot analysis of extracts from Rat lung using Histone H3 antibody.
Immunohistochemistry of paraffin-embedded rat brain using Histone H3 antibody.
Immunohistochemistry of paraffin-embedded human appendix using Histone H3 antibody.
Immunohistochemistry of paraffin-embedded mouse testis using Histone H3 antibody.
Immunohistochemistry of paraffin-embedded rat lung using Histone H3 Rabbit pAb.
Immunohistochemistry of paraffin-embedded human liver using Histone H3 Rabbit pAb.
Immunohistochemistry of paraffin-embedded human brain using Histone H3 Rabbit pAb.
Immunohistochemistry of paraffin-embedded mouse kidney using Histone H3 Rabbit pAb.
Immunofluorescence analysis of paraffin-embedded mouse testis using Histone H3 Antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of paraffin-embedded rat testis using Histone H3 Antibody at dilution of 1:200 (40x lens).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
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et al,Rho signalIng pathway enhances proliferation of PASMCs by suppressing nuclear translocation of Smad1 in PAH. In Exp Ther Med on 2019 Jan by Wei H, Zhang D, et al..PMID:30603049
, (2019),
PMID:
30603049
et al, Anthocyanins inhibit high-glucose-induced cholesterol accumulation and inflammation by activating LXR伪 pathway in HK-2 cells.In Drug Des Devel Ther on 2015 Sep 4 by Chunyang Du , Yonghong Shi et al..PMID:26379423
, (2015),
PMID:
26379423
et al,Sevoflurane preconditioning protects experimental ischemic stroke by enhancing anti-inflammatory microglia/macrophages phenotype polarization through GSK-3尾/Nrf2 pathway. In CNS Neurosci Ther on 2021 Nov by Min Cai, Sisi Sun, et al..PMID:34370899
, (2021),
PMID:
34370899
et al,Novel oxicam nonsteroidal compound XK01 attenuates inflammation by suppressing the NF-κB and MAPK pathway in RAW264.7 macrophages. In Heliyon on 2024 Jan 9 by Jixiang Wang, Jiawang Tan,et al..PMID:38312593
, (2024),
PMID:
38312593
et al,Dietary emulsifier Polysorbate 80-induced lipotoxicity promotes intestinal senescence
, (2025),
PMID:
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