产品详情
产品名称GOT1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB,IHC,IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total GOT1 protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant protein of human GOT1.
基因/蛋白名称GOT1
标记Unconjugated
别名cCAT; GIG18; cAspAT; ASTQTL1;
数据库入口号Swiss-Prot:P17174
NCBI Gene ID:2805
Uniprot
P17174
实际分子量46KD
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
Western blot analysis of extracts of various cell lines, using GOT1 antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat heart using GOT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using GOT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using GOT1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using GOT1 antibody. Blue: DAPI for nuclear staining.
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
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et al,c-Myc protects hepatocellular carcinoma cell from ferroptosis induced by glutamine deprivation via upregulating GOT1 and Nrf2InMol Biol RepOn2023 AugbyYuxiang Zhao?1,?Yue Wang et al..PMID:?37358765
, (2023),
PMID:
37358765
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