产品详情
产品名称PLCG1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous levels of total PLCG1 protein.
免疫原类型Peptide
免疫原描述Synthesized non-phosphopeptide derived from human PLCG1 around the phosphorylation site of tyrosine 771 (P-D-Y-G-A).
基因/蛋白名称PLCG1
标记Unconjugated
别名Phospholipase C gamma 1; PLC148; PHOSPHOLIPASE C-148;
数据库入口号Swiss-Prot: P19174
NCBI Gene ID: 5335
Uniprot
P19174
实际分子量150kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PLCG1 (Ab-771) antibody #33125.
Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30min), using PLCG1 (Ab-771) antibody #33125.
Western blot analysis of extracts from COS7 cells (Lane 2), and JK cells (Lane 3), using PLCG1 (Ab-771) antiobdy #33125. The lane on the left is treated with synthesized peptide.
Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Denis Tvorogov, J. Cell Sci., Feb 2005; 118: 601 - 610.
Brenda J. Irvin, Mol. Cell. Biol., Dec 2000; 20: 9149 - 9161.
Benoit Poulin, J. Biol. Chem., Feb 2000; 275: 6411.
Ichiro Nakamura, J. Cell Biol., Jan 2001; 152: 361.
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et al,Discovery of a Novel Src Homology-2 Domain Containing Protein Tyrosine Phosphatase-2 (SHP2) and Cyclin-Dependent Kinase 4 (CDK4) Dual Inhibitor for the Treatment of Triple-Negative Breast Cancer
, (2022),
PMID:
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