产品详情
产品名称4E-BP1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous levels of total 4E-BP1 protein.
免疫原类型Peptide
免疫原描述Synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of serine 65 (R-N-S(p)-P-V).
基因/蛋白名称4E-BP1
标记Unconjugated
别名4EBP1; EIF4EBP1; P/OKCL.6; PHAS-1;
数据库入口号Swiss-Prot: Q13541
NCBI Gene ID: 1978
Uniprot
Q13541
实际分子量15kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01U/ml, 15mins), using 4E-BP1 (Ab-64) antibody #33127.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Ab-64) antibody #33127.
Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Anne-Claude Gingras, Genes & Dev., Jun 1999; 13: 1422.
Guangming Liu, J. Biol. Chem., Mar 2002; 277: 8810 - 8816.
Ya-Ping Jiang, J. Biol. Chem., Mar 2001; 276: 10943 - 10951.
如果您使用该产品33127发表了文章，请通知我们，让我们可以引用您的文献。
et al,Monotropein inhibits epithelial-mesenchymal transition in chronic colitis via the mTOR/P70S6K pathway.
, (2025),
PMID:
40041493
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