产品详情
产品名称TTK Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC
种属反应性Human
特异性The antibody detects endogenous levels of total TTK protein.
免疫原类型Peptide
免疫原描述Synthesized non-phosphopeptide derived from human TTK around the phosphorylation site of threonine 676 (D-T-T(p)-S-V).
基因/蛋白名称TTK
标记Unconjugated
别名dual specificity protein kinase TTK; EC 2.7.12.1; Esk1; Mps1; MPS1L1
数据库入口号Swiss-Prot: P33981
NCBI Gene ID: 7272
Uniprot
P33981
实际分子量100kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunohistochemistry analysis of paraffin-embedded human brain tissue using TTK (Ab-676) antibody #33252.
Western blot analysis of extracts from HUVEC cells, treated with etoposide (25uM, 24hours), using TTK (Ab-676) antibody #33252.
Western blot analysis of extracts from colo cells (Lane 2), using TTK (Ab-676) antiobdy #33252. The lane on the left is treated with synthesized peptide.
Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
Mills G.B., J. Biol. Chem. 267:16000-16006(1992).
Ota T., Nat. Genet. 36:40-45(2004).
Mungall A.J., Nature 425:805-811(2003)
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