产品详情
产品名称p47 phox Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC
种属反应性Human
特异性The antibody detects endogenous levels of total p47 phox protein.
免疫原类型Peptide
免疫原描述Synthesized non-phosphopeptide derived from human p47 phox around the phosphorylation site of serine 345 (P-Q-S(p)-P-G).
基因/蛋白名称p47 phox
标记Unconjugated
别名47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-1; NCF-47K; NCF1
数据库入口号Swiss-Prot: P14598
NCBI Gene ID: 653361
Uniprot
P14598
实际分子量45kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HepG2 cells, treated with TNF (20ng/ml, 5mins), using p47 phox (Ab-345) antibody #33293.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using p47 phox (Ab-345) antibody #33293.
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).
Lomax K.J., Science 245:409-412(1989).
Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).
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et al,Clonorchis sinensis ferritin heavy chain triggers free radicals and mediates inflammation signaling in human hepatic stellate cells.In Parasitol Res on 2015 Feb by Qiang Mao, Zhizhi Xie et al..PMID: 25413629
, (2015),
PMID:
25413629
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